BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 53 52 40.59 0.66 2 2 23 21 43.15 0.61 3 2 24 22 42.07 0.37 4 2 34 32 43.10 0.60 5 2 57 55 37.65 1.57 6 3 21 18 43.20 0.58 7 3 23 20 43.16 0.60 8 4 18 14 43.09 0.63 9 4 19 15 43.14 0.61 10 4 20 16 43.15 0.60 11 4 54 50 43.12 0.61 12 4 57 53 43.12 0.61 13 4 58 54 43.01 0.67 14 5 18 13 43.14 0.61 15 5 19 14 43.14 0.61 16 5 21 16 43.88 0.29 17 5 152 147 59.51 1.12 18 5 155 150 45.27 0.06 19 5 215 210 60.22 1.36 20 5 224 219 44.78 0.50 21 7 17 10 43.52 0.43 22 7 18 11 43.62 0.40 23 7 111 104 43.60 0.40 24 7 152 145 56.58 1.70 25 8 152 144 61.05 0.94 26 8 211 203 61.05 0.94 27 9 17 8 398.79 103.56 28 9 111 102 61.05 0.94 29 9 113 104 61.02 0.93 30 9 150 141 61.02 0.93 31 9 151 142 61.05 0.94 32 9 152 143 61.06 0.95 33 9 211 202 61.06 0.94 34 9 240 231 61.01 0.96 35 10 150 140 61.04 0.93 36 10 211 201 62.97 0.35 37 11 15 4 793.11 6.21 38 11 16 5 763.01 7.32 39 11 17 6 529.88 43.63 40 12 15 3 824.03 3.53 41 12 113 101 61.08 0.93 42 12 114 102 61.06 0.92 43 12 115 103 61.06 0.92 44 12 117 105 60.98 0.94 45 12 150 138 62.78 0.29 46 13 243 230 60.91 0.96 47 14 242 228 61.00 0.94 48 14 243 229 60.75 0.88 49 15 113 98 62.99 0.36 50 15 117 102 62.70 0.35 51 15 120 105 62.94 0.37 52 15 240 225 62.99 0.36 53 15 241 226 61.11 0.94 54 15 242 227 61.05 0.95 55 15 243 228 61.01 0.94 56 16 240 224 63.00 0.36 57 16 241 225 62.81 0.30 58 16 243 227 61.05 0.92 59 17 111 94 64.94 0.09 60 17 239 222 63.69 0.56 61 17 240 223 63.00 0.36 62 17 241 224 63.00 0.36 63 18 58 40 66.72 0.02 64 18 60 42 66.56 0.10 65 18 238 220 69.39 1.28 66 18 239 221 63.83 0.56 67 18 241 223 65.81 0.47 68 19 155 136 73.80 0.39 69 19 237 218 73.00 0.71 70 19 238 219 70.92 1.26 71 20 34 14 103.19 0.21 72 20 36 16 97.34 0.49 73 20 54 34 74.11 0.58 74 20 236 216 73.12 0.71 75 20 237 217 73.12 0.71 76 21 34 13 103.71 0.46 77 21 223 202 105.33 0.38 78 21 224 203 104.00 0.21 79 21 236 215 97.78 0.69 80 22 32 10 108.05 0.45 81 22 33 11 107.76 0.47 82 22 34 12 103.17 0.20 83 22 221 199 107.86 0.43 84 22 222 200 107.89 0.42 85 22 223 201 106.68 0.03 86 22 224 202 104.50 0.04 87 22 225 203 104.50 0.05 88 22 229 207 108.21 0.44 89 23 33 10 107.98 0.48 90 23 34 11 107.64 0.48 91 23 222 199 108.12 0.48 92 23 223 200 107.78 0.43 93 24 33 9 108.48 0.56 94 24 34 10 107.88 0.42 95 24 53 29 107.80 0.45 96 25 33 8 108.48 0.56 97 25 34 9 108.00 0.47 98 25 50 25 107.92 0.51 99 25 53 28 108.00 0.48 100 26 33 7 110.36 0.33 101 26 46 20 110.86 0.55 102 26 49 23 110.34 0.29 103 26 50 24 109.33 0.18 104 27 31 4 747.82 18.90 105 27 32 5 624.75 5.00 106 27 33 6 533.40 39.23 107 27 35 8 114.61 1.33 108 27 42 15 129.56 0.78 109 27 46 19 114.66 1.30 110 27 50 23 110.86 0.55 111 28 31 3 858.85 0.16 112 28 32 4 708.28 31.40 113 28 46 18 130.35 1.18 114 29 32 3 817.10 1.26 115 30 232 202 153.28 1.06 116 30 233 203 143.22 0.32 117 31 35 4 712.09 18.96 118 31 40 9 155.81 0.79 119 31 41 10 155.85 0.80 120 31 42 11 155.82 0.80 121 31 225 194 143.96 0.69 122 31 232 201 155.56 0.96 123 31 233 202 149.52 2.52 124 31 234 203 138.88 0.35 125 32 225 193 155.31 0.88 126 33 50 17 155.44 0.97 127 34 50 16 155.44 0.97 128 34 54 20 153.37 1.33 129 34 225 191 155.68 0.91 130 35 40 5 813.51 2.71 131 35 42 7 156.00 0.80 132 35 47 12 155.92 0.81 133 35 50 15 155.88 0.82 134 35 225 190 155.69 0.92 135 35 234 199 155.68 0.93 136 35 235 200 155.62 0.93 137 36 47 11 155.95 0.80 138 36 50 14 155.96 0.80 139 36 51 15 155.93 0.79 140 36 54 18 155.85 0.83 141 36 66 30 155.59 0.96 142 36 103 67 155.63 0.94 143 36 235 199 155.68 0.93 144 36 237 201 155.60 0.94 145 36 239 203 155.56 0.95 146 37 40 3 862.10 1.39 147 37 47 10 156.00 0.79 148 37 103 66 155.59 0.97 149 37 235 198 155.62 0.96 150 38 47 9 156.08 0.77 151 38 51 13 156.08 0.77 152 40 233 193 155.97 1.14 153 40 234 194 155.70 1.02 154 40 235 195 155.70 1.02 155 42 47 5 798.87 3.39 156 43 46 3 828.53 3.85 157 43 47 4 816.60 0.56 158 44 47 3 828.53 3.85 159 44 48 4 825.85 5.19 160 45 48 3 833.89 1.17 161 45 49 4 816.62 0.56 162 46 49 3 816.62 0.56 163 46 50 4 816.62 0.56 164 47 50 3 824.56 4.53 165 47 51 4 822.16 4.20 166 48 51 3 823.03 3.77 167 48 52 4 822.13 4.21 168 49 52 3 819.07 5.75 169 49 53 4 818.62 5.97 170 50 53 3 830.50 1.40 171 50 54 4 818.64 5.99 172 51 54 3 830.53 1.42 173 51 55 4 818.62 6.00 174 51 66 15 178.75 1.86 175 51 103 52 179.06 1.97 176 52 55 3 841.38 4.00 177 52 56 4 820.07 6.65 178 53 56 3 843.50 5.06 179 53 57 4 818.70 5.96 180 54 57 3 830.86 0.12 181 54 58 4 818.68 5.96 182 54 239 185 178.72 1.97 183 55 58 3 833.05 0.98 184 55 59 4 805.13 2.21 185 55 60 5 771.86 5.07 186 55 64 9 185.58 1.45 187 55 66 11 185.97 1.25 188 56 59 3 820.71 6.44 189 58 241 183 179.11 1.99 190 59 241 182 179.18 1.99 191 60 64 4 840.05 0.35 192 60 99 39 179.41 2.08 193 60 239 179 179.47 2.06 194 60 241 181 179.15 2.00 195 61 97 36 179.16 2.00 196 61 99 38 179.60 2.00 197 61 241 180 179.44 2.06 198 62 97 35 179.58 2.00 199 62 99 37 179.60 2.00 200 64 99 35 182.78 0.54 201 64 101 37 186.38 0.68 202 64 239 175 183.96 0.52 203 65 78 13 192.07 2.65 204 65 83 18 244.33 3.71 205 65 98 33 188.43 1.75 206 65 99 34 188.43 1.75 207 66 86 20 244.87 3.91 208 66 101 35 200.81 0.29 209 66 103 37 218.58 4.09 210 66 237 171 214.09 6.34 211 66 239 173 202.71 0.66 212 67 76 9 244.43 3.93 213 67 86 19 244.87 3.91 214 67 88 21 244.88 3.89 215 67 89 22 244.70 3.92 216 67 90 23 243.70 3.57 217 67 91 24 235.75 0.86 218 68 86 18 244.93 3.93 219 68 87 19 244.93 3.92 220 68 88 20 244.94 3.91 221 69 88 19 245.00 3.89 222 69 89 20 244.88 3.90 223 69 102 33 244.93 3.86 224 69 103 34 244.95 3.85 225 69 124 55 244.97 3.85 226 70 87 17 283.09 4.14 227 70 89 19 254.33 0.74 228 71 89 18 261.19 1.41 229 71 107 36 277.79 0.99 230 71 124 53 260.22 0.76 231 72 89 17 291.89 1.09 232 72 129 57 297.56 2.64 233 73 129 56 297.73 2.63 234 73 132 59 297.95 2.60 235 73 133 60 297.88 2.60 236 73 134 61 297.62 2.62 237 73 137 64 297.78 2.63 238 74 88 14 297.80 2.63 239 74 89 15 297.80 2.64 240 74 90 16 297.78 2.64 241 75 90 15 297.78 2.64 242 75 91 16 297.78 2.63 243 75 92 17 297.78 2.64 244 75 137 62 298.05 2.56 245 75 138 63 297.80 2.64 246 75 141 66 297.81 2.62 247 76 83 7 529.52 44.36 248 76 86 10 298.13 2.56 249 76 88 12 298.09 2.56 250 76 90 14 297.88 2.60 251 76 91 15 297.80 2.64 252 76 92 16 297.80 2.64 253 77 83 6 798.30 3.84 254 77 92 15 297.80 2.64 255 77 141 64 298.06 2.56 256 78 83 5 818.27 0.42 257 78 91 13 298.05 2.56 258 78 92 14 297.98 2.59 259 78 93 15 297.82 2.63 260 78 98 20 297.79 2.64 261 78 100 22 297.80 2.64 262 79 83 4 857.02 1.55 263 80 83 3 885.93 1.26 264 83 86 3 879.80 1.84 265 89 123 34 355.32 1.92 266 89 124 35 355.32 1.92 267 90 122 32 355.38 1.90 268 90 123 33 355.33 1.92 269 90 134 44 356.60 1.64 270 90 138 48 356.75 1.55 271 91 100 9 356.56 1.61 272 91 121 30 356.70 1.54 273 91 122 31 356.54 1.62 274 92 100 8 356.88 1.49 275 92 119 27 357.33 1.28 276 92 120 28 357.26 1.30 277 92 121 29 356.86 1.50 278 92 141 49 357.36 1.36 279 92 142 50 357.42 1.32 280 93 98 5 621.40 4.17 281 93 100 7 357.25 1.30 282 93 119 26 357.33 1.28 283 93 120 27 357.27 1.30 284 94 118 24 393.61 0.77 285 94 119 25 359.70 0.09 286 94 242 148 355.30 1.95 287 95 98 3 836.96 0.78 288 95 242 147 355.32 1.94 289 96 242 146 355.33 1.94 290 96 243 147 355.32 1.94 291 97 242 145 355.39 1.97 292 98 241 143 355.42 1.96 293 99 239 140 355.68 1.89 294 99 240 141 355.62 1.88 295 99 241 142 355.35 1.97 296 100 120 20 427.99 1.81 297 100 121 21 428.64 1.49 298 100 122 22 428.65 1.49 299 100 239 139 355.63 1.88 300 100 240 140 355.40 1.97 301 101 122 21 429.15 1.60 302 101 238 137 355.69 1.88 303 101 239 138 355.62 1.85 304 102 109 7 581.02 77.53 305 102 122 20 429.23 1.64 306 102 123 21 429.23 1.64 307 102 124 22 506.91 40.48 308 102 237 135 355.72 1.89 309 102 238 136 355.72 1.89 310 103 124 21 513.92 36.99 311 103 235 132 357.40 2.27 312 103 236 133 357.25 2.33 313 103 237 134 355.71 1.88 314 104 107 3 838.62 8.24 315 104 109 5 594.72 70.75 316 104 124 20 525.61 31.16 317 104 235 131 357.41 2.26 318 104 236 132 357.34 2.28 319 105 225 120 409.77 1.53 320 105 226 121 407.10 2.83 321 105 227 122 407.12 2.85 322 105 234 129 358.82 2.22 323 105 235 130 357.42 2.26 324 105 236 131 357.39 2.25 325 106 156 50 536.31 0.51 326 106 157 51 534.73 0.08 327 106 217 111 424.29 1.20 328 106 226 120 409.77 1.53 329 106 227 121 408.83 2.00 330 106 228 122 407.13 2.85 331 107 124 17 735.05 0.10 332 107 126 19 735.04 0.10 333 107 156 49 537.27 0.66 334 108 123 15 735.33 0.05 335 108 124 16 735.10 0.11 336 108 125 17 735.06 0.10 337 108 153 45 541.42 0.42 338 108 154 46 539.81 0.71 339 108 156 48 537.32 0.64 340 108 194 86 536.92 0.75 341 108 195 87 536.88 0.77 342 108 198 90 536.87 0.78 343 109 123 14 735.33 0.05 344 109 153 44 541.48 0.42 345 109 154 45 539.82 0.71 346 109 155 46 539.82 0.71 347 109 156 47 539.66 0.64 348 109 238 129 355.58 1.90 349 110 122 12 735.56 0.07 350 110 123 13 735.35 0.04 351 110 135 25 735.23 0.09 352 110 151 41 546.96 0.24 353 110 152 42 544.62 0.96 354 110 153 43 541.62 0.35 355 111 121 10 735.69 0.07 356 111 122 11 735.57 0.07 357 111 151 40 547.50 0.48 358 111 152 41 546.84 0.18 359 111 238 127 355.72 1.89 360 111 239 128 355.66 1.86 361 111 240 129 355.39 1.96 362 112 120 8 735.75 0.06 363 112 121 9 735.70 0.08 364 112 123 11 735.68 0.07 365 112 135 23 735.31 0.08 366 112 138 26 735.19 0.09 367 112 139 27 735.09 0.12 368 112 142 30 734.88 0.07 369 112 149 37 548.76 0.18 370 112 150 38 548.54 0.14 371 112 151 39 547.53 0.48 372 112 202 90 537.74 0.51 373 112 240 128 355.41 1.96 374 113 119 6 735.98 0.10 375 113 120 7 735.75 0.06 376 113 142 29 734.96 0.09 377 113 149 36 548.82 0.21 378 113 150 37 548.74 0.18 379 113 240 127 355.42 1.96 380 114 117 3 816.52 13.04 381 114 118 4 778.68 4.95 382 114 119 5 736.03 0.12 383 114 142 28 735.00 0.07 384 114 144 30 733.61 0.42 385 114 148 34 581.72 16.18 386 114 149 35 548.82 0.18 387 115 148 33 600.61 6.75 388 115 150 35 548.86 0.17 389 115 209 94 525.54 0.15 390 117 242 125 355.38 1.97 391 118 242 124 355.37 1.97 392 119 142 23 735.00 0.11 393 119 143 24 734.97 0.10 394 119 144 25 734.82 0.04 395 119 242 123 355.26 1.95 396 120 142 22 734.98 0.10 397 120 240 120 355.39 1.96 398 120 241 121 355.36 1.97 399 120 242 122 355.25 1.93 400 121 138 17 735.22 0.10 401 121 142 21 734.94 0.10 402 121 240 119 355.38 1.96 403 122 240 118 355.32 1.99 404 123 134 11 735.56 0.23 405 123 135 12 735.52 0.23 406 123 138 15 735.08 0.12 407 125 130 5 772.39 8.21 408 125 134 9 735.77 0.32 409 125 194 69 546.65 0.14 410 126 129 3 837.93 2.68 411 126 130 4 812.39 1.58 412 127 130 3 813.11 1.23 413 127 131 4 813.10 1.24 414 127 162 35 547.96 0.55 415 127 168 41 547.98 0.55 416 127 171 44 547.97 0.55 417 127 172 45 547.94 0.56 418 128 131 3 836.84 3.85 419 128 132 4 812.39 1.59 420 129 132 3 822.64 2.15 421 129 133 4 812.39 1.59 422 129 134 5 788.35 10.96 423 130 133 3 838.32 9.38 424 130 134 4 807.22 1.66 425 130 135 5 807.21 1.66 426 130 172 42 547.71 0.64 427 130 194 64 546.86 0.24 428 130 197 67 544.85 1.02 429 130 198 68 544.79 1.02 430 130 201 71 544.68 0.98 431 131 171 40 547.93 0.54 432 131 172 41 547.68 0.65 433 131 175 44 547.73 0.62 434 131 201 70 544.74 0.99 435 133 136 3 841.69 0.05 436 133 137 4 822.84 1.73 437 133 201 68 543.48 0.72 438 134 137 3 834.69 3.54 439 134 138 4 822.83 1.72 440 135 138 3 834.03 3.88 441 135 139 4 834.02 3.88 442 135 198 63 544.38 1.07 443 135 202 67 538.82 0.06 444 136 139 3 836.68 2.84 445 136 140 4 825.38 3.02 446 136 201 65 539.83 0.12 447 136 205 69 528.45 0.55 448 137 140 3 836.89 2.73 449 137 141 4 836.88 2.73 450 138 141 3 838.74 3.31 451 138 142 4 837.23 2.56 452 139 142 3 837.24 2.56 453 139 146 7 597.81 7.73 454 139 149 10 548.89 0.23 455 139 202 63 538.64 0.14 456 139 205 66 529.22 0.16 457 139 207 68 527.71 0.82 458 142 149 7 606.72 3.69 459 144 148 4 823.48 2.48 460 145 148 3 848.98 1.49 461 145 149 4 827.62 0.77 462 146 149 3 840.57 5.70 463 146 205 59 534.58 0.72 464 146 206 60 528.28 0.55 465 146 207 61 527.70 0.82 466 147 209 62 527.64 0.84 467 149 207 58 527.36 0.95 468 149 209 60 497.40 0.07 469 150 209 59 465.53 0.89 470 150 211 61 442.32 2.43 471 151 202 51 537.33 0.68 472 151 207 56 511.00 0.56 473 151 209 58 455.44 4.12 474 151 210 59 440.77 2.97 475 151 211 60 440.75 2.96 476 152 211 59 433.05 0.38 477 152 212 60 432.50 0.14 478 153 195 42 542.84 2.04 479 153 198 45 540.62 0.42 480 153 199 46 540.11 0.69 481 153 210 57 440.75 2.96 482 153 211 58 433.10 0.24 483 153 212 59 428.41 1.85 484 153 213 60 427.91 1.61 485 154 195 41 548.79 0.96 486 155 195 40 549.12 1.05 487 155 215 60 426.35 1.50 488 155 217 62 425.73 1.36 489 155 224 69 410.12 1.69 490 156 194 38 549.38 1.17 491 156 195 39 549.13 1.06 492 156 215 59 426.42 1.49 493 156 216 60 425.93 1.39 494 156 217 61 425.71 1.37 495 157 161 4 777.04 9.33 496 157 162 5 599.21 7.13 497 157 194 37 549.41 1.18 498 157 216 59 426.32 1.52 499 157 217 60 425.88 1.42 500 157 218 61 425.74 1.35 501 157 228 71 410.00 1.63 502 158 161 3 843.33 5.33 503 158 162 4 770.28 7.15 504 158 195 37 549.43 1.17 505 158 216 58 426.46 1.51 506 159 191 32 550.64 0.59 507 159 192 33 549.47 1.17 508 159 193 34 549.44 1.17 509 159 195 36 549.46 1.17 510 160 191 31 551.85 0.77 511 161 216 55 427.06 1.78 512 161 218 57 426.23 1.52 513 161 228 67 410.34 1.75 514 162 191 29 584.61 5.37 515 162 192 30 563.23 4.94 516 162 194 32 553.64 0.36 517 162 228 66 410.30 1.75 518 163 186 23 592.23 6.59 519 163 189 26 592.29 6.57 520 163 190 27 591.89 6.65 521 163 191 28 585.96 5.42 522 163 228 65 410.32 1.75 523 164 168 4 826.28 0.57 524 164 183 19 592.11 6.58 525 164 189 25 592.32 6.57 526 164 190 26 592.21 6.61 527 164 192 28 591.10 6.84 528 165 168 3 834.87 0.14 529 165 169 4 827.36 0.70 530 165 189 24 592.32 6.57 531 166 169 3 838.69 4.79 532 166 170 4 827.76 0.17 533 166 190 24 592.45 6.51 534 167 170 3 830.77 1.68 535 167 171 4 823.89 2.17 536 168 171 3 830.75 1.69 537 168 172 4 826.97 3.58 538 169 172 3 843.12 0.32 539 169 173 4 825.47 2.82 540 169 183 14 592.47 6.50 541 169 190 21 592.62 6.47 542 170 173 3 850.60 2.27 543 170 174 4 824.12 2.15 544 171 174 3 833.85 1.93 545 171 175 4 830.55 0.28 546 172 175 3 852.73 3.98 547 172 176 4 824.13 2.16 548 172 179 7 602.46 1.62 549 172 183 11 592.72 6.49 550 172 197 25 592.45 6.56 551 172 201 29 592.34 6.59 552 173 176 3 826.26 2.31 553 173 177 4 767.83 5.79 554 173 179 6 601.74 4.82 555 173 180 7 598.91 3.40 556 175 201 26 592.34 6.61 557 176 179 3 861.12 0.59 558 176 204 28 592.14 6.50 559 177 180 3 858.71 1.31 560 178 181 3 861.25 0.70 561 178 200 22 592.56 6.52 562 179 182 3 844.60 4.51 563 179 183 4 829.43 3.07 564 179 197 18 592.68 6.55 565 179 200 21 592.56 6.52 566 180 183 3 848.25 2.68 567 181 196 15 593.53 6.29 568 182 191 9 595.36 6.45 569 182 192 10 593.63 6.29 570 182 193 11 593.58 6.30 571 182 196 14 593.53 6.29 572 182 197 15 593.47 6.31 573 183 190 7 595.58 6.54 574 183 191 8 595.34 6.46 575 183 192 9 593.99 6.12 576 183 197 14 593.60 6.31 577 184 190 6 595.68 6.50 578 184 191 7 595.52 6.54 579 185 189 4 754.39 2.25 580 185 190 5 600.05 4.33 581 186 189 3 812.80 0.35 582 186 190 4 609.07 6.30 583 186 191 5 602.62 3.08 584 193 196 3 825.63 5.26 585 193 197 4 804.19 2.78 586 194 197 3 812.56 0.64 587 194 198 4 808.09 0.87 588 195 198 3 840.40 2.12 589 195 199 4 828.26 8.19 590 195 213 18 428.02 1.67 591 195 215 20 427.95 1.64 592 196 199 3 834.05 5.30 593 196 200 4 828.26 8.19 594 197 200 3 838.16 3.24 595 197 201 4 840.39 2.12 596 198 201 3 840.39 2.13 597 198 202 4 826.80 1.67 598 199 202 3 831.74 4.13 599 199 203 4 821.62 4.25 600 199 210 11 441.03 2.85 601 199 212 13 441.01 2.85 602 200 203 3 831.21 3.87 603 200 204 4 826.02 6.46 604 201 204 3 837.05 0.95 605 201 205 4 826.02 6.46 606 202 205 3 837.09 0.93 607 202 206 4 826.00 6.46 608 202 207 5 731.13 22.29 609 202 210 8 461.04 1.28 610 203 206 3 830.37 3.45 611 207 210 3 908.13 2.21 612 215 220 5 623.78 6.28 613 215 223 8 521.08 52.16 614 215 224 9 413.79 1.49 615 216 219 3 829.98 5.34 616 216 220 4 698.16 23.45 617 216 224 8 519.57 52.84 618 217 224 7 410.25 1.73 619 217 225 8 410.24 1.74 620 217 226 9 410.19 1.73 621 217 229 12 410.12 1.73 622 217 236 19 358.86 2.24 623 218 226 8 410.22 1.73 624 218 228 10 410.20 1.72 625 218 229 11 410.06 1.71 626 220 223 3 835.49 1.61 627 220 224 4 804.94 3.54 628 221 224 3 839.85 5.83 629 221 229 8 411.31 1.30 630 224 229 5 836.27 4.30 631 224 236 12 359.00 2.31 632 225 229 4 850.31 2.71 633 225 233 8 359.56 2.23 634 225 234 9 359.41 2.26 635 225 235 10 359.24 2.23 636 225 236 11 359.01 2.31 637 226 229 3 826.78 1.26 638 226 230 4 814.99 4.62 639 226 233 7 475.63 60.06 640 227 231 4 722.51 36.54 641 227 233 6 618.28 2.83 642 227 235 8 475.44 60.15 643 230 233 3 844.30 2.94