BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 32 30 702.96 0.12 2 2 33 31 702.89 0.14 3 2 34 32 702.88 0.14 4 2 35 33 701.52 0.58 5 2 36 34 687.41 2.06 6 2 176 174 265.45 0.57 7 3 34 31 702.90 0.14 8 3 176 173 265.68 0.53 9 3 180 177 265.44 0.56 10 3 243 240 22.57 0.11 11 4 34 30 703.02 0.11 12 4 35 31 702.90 0.14 13 4 36 32 702.90 0.14 14 4 176 172 265.77 0.54 15 4 180 176 265.44 0.56 16 4 182 178 265.44 0.56 17 4 242 238 24.10 0.76 18 4 243 239 23.86 0.74 19 5 36 31 702.91 0.14 20 5 170 165 267.50 0.99 21 5 171 166 267.43 1.02 22 5 174 169 266.78 0.73 23 5 176 171 265.80 0.54 24 5 180 175 265.56 0.56 25 5 181 176 265.44 0.56 26 5 182 177 265.44 0.56 27 6 14 8 708.00 0.24 28 6 28 22 703.35 0.17 29 6 36 30 703.02 0.11 30 6 37 31 702.94 0.13 31 6 38 32 702.93 0.12 32 6 182 176 265.44 0.56 33 6 184 178 265.44 0.56 34 6 233 227 70.65 1.06 35 6 237 231 54.28 0.44 36 7 38 31 702.94 0.13 37 7 40 33 702.91 0.14 38 7 64 57 702.90 0.14 39 7 167 160 268.65 1.35 40 7 170 163 268.62 1.35 41 7 181 174 265.63 0.54 42 7 182 175 265.56 0.56 43 7 183 176 265.45 0.57 44 7 184 177 265.45 0.57 45 7 193 186 198.01 0.53 46 8 13 5 739.76 16.11 47 8 14 6 708.00 0.24 48 8 38 30 703.01 0.11 49 8 39 31 702.99 0.11 50 8 40 32 702.98 0.11 51 8 184 176 265.45 0.57 52 8 233 225 70.67 1.05 53 9 13 4 758.00 25.23 54 9 14 5 739.76 16.11 55 9 40 31 703.00 0.10 56 9 163 154 269.91 1.56 57 9 184 175 265.58 0.55 58 9 185 176 265.47 0.56 59 9 186 177 265.47 0.56 60 9 190 181 198.19 0.55 61 10 13 3 834.88 5.77 62 10 14 4 758.00 25.23 63 10 15 5 708.02 0.24 64 10 39 29 703.04 0.10 65 10 41 31 703.00 0.10 66 10 48 38 703.01 0.11 67 10 51 41 703.03 0.10 68 10 57 47 703.49 0.20 69 11 15 4 708.03 0.24 70 11 43 32 703.02 0.10 71 11 186 175 265.56 0.56 72 12 15 3 846.99 1.59 73 12 186 174 265.56 0.56 74 12 203 191 197.19 0.58 75 12 205 193 193.44 0.12 76 12 229 217 77.75 0.20 77 13 184 171 265.62 0.55 78 13 185 172 265.56 0.56 79 13 186 173 265.46 0.56 80 13 203 190 196.69 0.68 81 13 229 216 77.63 0.21 82 13 230 217 77.56 0.17 83 13 233 220 76.26 0.09 84 14 21 7 703.92 0.23 85 14 24 10 704.03 0.18 86 14 25 11 703.95 0.22 87 14 28 14 703.96 0.22 88 14 37 23 703.25 0.15 89 14 39 25 703.09 0.12 90 14 57 43 703.49 0.21 91 14 58 44 703.54 0.20 92 14 230 216 77.35 0.10 93 14 233 219 72.14 0.45 94 15 21 6 704.39 0.35 95 15 51 36 703.26 0.10 96 15 55 40 703.49 0.21 97 15 57 42 703.49 0.21 98 15 229 214 77.70 0.21 99 16 229 213 77.74 0.20 100 17 20 3 846.60 0.45 101 17 21 4 814.47 2.71 102 17 229 212 77.75 0.20 103 18 21 3 825.27 3.62 104 18 22 4 826.95 1.54 105 18 55 37 703.83 0.10 106 19 22 3 853.66 4.92 107 19 23 4 826.96 1.54 108 20 23 3 829.76 2.94 109 20 24 4 826.96 1.54 110 20 230 210 77.50 0.17 111 20 231 211 77.34 0.11 112 21 24 3 840.20 3.52 113 21 25 4 826.97 1.54 114 21 55 34 703.89 0.07 115 21 57 36 703.92 0.09 116 21 230 209 77.34 0.11 117 22 25 3 849.51 3.09 118 22 26 4 832.50 11.60 119 23 26 3 854.28 0.70 120 23 27 4 821.87 6.82 121 23 234 211 71.64 0.82 122 24 27 3 847.48 2.08 123 24 28 4 832.79 1.36 124 24 230 206 77.33 0.11 125 24 233 209 71.69 0.80 126 24 234 210 71.02 0.80 127 24 237 213 66.97 0.24 128 25 28 3 854.41 1.39 129 25 29 4 836.47 0.49 130 25 56 31 704.51 0.12 131 25 57 32 704.10 0.07 132 25 58 33 704.04 0.10 133 26 29 3 837.32 0.84 134 26 30 4 836.48 0.50 135 27 30 3 862.33 0.30 136 27 31 4 838.31 0.35 137 27 234 207 71.02 0.80 138 27 237 210 63.36 0.77 139 27 243 216 25.98 0.21 140 28 31 3 848.14 4.32 141 28 32 4 830.35 2.54 142 28 35 7 807.70 6.12 143 28 58 30 721.42 4.46 144 28 237 209 59.47 0.01 145 28 243 215 24.28 0.76 146 29 32 3 847.90 4.50 147 29 58 29 710.12 2.75 148 31 34 3 864.26 2.82 149 31 35 4 860.89 1.36 150 31 243 212 24.03 0.73 151 32 35 3 871.57 3.41 152 32 37 5 840.51 1.36 153 32 58 26 756.00 0.12 154 32 59 27 756.11 0.16 155 32 60 28 756.13 0.17 156 32 61 29 756.09 0.18 157 34 243 209 23.09 0.37 158 35 58 23 780.81 5.17 159 35 243 208 23.88 0.72 160 36 61 25 780.58 5.21 161 36 174 138 266.77 0.74 162 36 176 140 265.82 0.55 163 37 58 21 780.81 5.17 164 37 60 23 780.72 5.18 165 37 61 24 780.70 5.17 166 37 62 25 780.61 5.21 167 38 60 22 780.77 5.17 168 38 62 24 780.59 5.21 169 38 64 26 780.60 5.21 170 38 170 132 268.52 1.37 171 39 57 18 781.00 5.12 172 39 58 19 781.00 5.12 173 39 60 21 780.82 5.15 174 39 62 23 780.76 5.16 175 39 63 24 780.74 5.16 176 39 64 25 780.61 5.21 177 40 64 24 780.62 5.21 178 40 163 123 268.69 1.39 179 40 193 153 198.09 0.55 180 41 48 7 781.23 5.25 181 41 64 23 780.75 5.17 182 41 65 24 780.75 5.17 183 41 66 25 780.64 5.21 184 41 163 122 276.28 1.60 185 42 66 24 780.74 5.16 186 42 67 25 780.57 5.21 187 43 47 4 810.57 6.72 188 43 48 5 806.78 4.87 189 43 51 8 794.98 4.61 190 43 65 22 780.82 5.17 191 43 66 23 780.77 5.16 192 44 47 3 832.88 4.44 193 44 48 4 823.86 0.07 194 44 65 21 780.84 5.16 195 44 66 22 780.80 5.15 196 45 48 3 839.63 1.06 197 45 49 4 833.63 4.07 198 45 63 18 781.06 5.12 199 45 65 20 780.84 5.16 200 45 72 27 362.16 0.50 201 46 49 3 839.63 1.06 202 46 50 4 826.88 0.69 203 47 50 3 834.57 2.56 204 47 51 4 822.27 0.46 205 48 51 3 835.50 2.09 206 48 52 4 822.27 0.46 207 48 63 15 781.05 5.11 208 49 52 3 831.84 4.32 209 49 53 4 831.82 4.33 210 49 59 10 781.38 5.16 211 49 63 14 781.11 5.12 212 50 53 3 837.76 2.04 213 50 54 4 831.82 4.33 214 51 54 3 831.42 5.21 215 51 55 4 830.74 4.87 216 51 57 6 798.84 1.27 217 52 55 3 840.05 0.22 218 52 56 4 809.18 2.71 219 52 57 5 785.78 2.96 220 52 59 7 785.77 2.96 221 52 63 11 781.14 5.11 222 60 63 3 895.08 1.94 223 61 73 12 361.58 0.55 224 61 74 13 361.58 0.55 225 62 71 9 361.88 0.48 226 62 72 10 361.69 0.51 227 62 73 11 361.58 0.55 228 62 170 108 267.88 1.22 229 62 173 111 267.44 1.01 230 62 174 112 267.30 0.95 231 63 71 8 361.89 0.48 232 63 72 9 361.69 0.51 233 64 70 6 362.03 0.44 234 64 71 7 361.89 0.48 235 64 166 102 268.76 1.32 236 64 167 103 268.77 1.32 237 64 170 106 268.62 1.35 238 65 68 3 837.41 4.74 239 65 69 4 811.52 4.91 240 65 70 5 362.10 0.47 241 66 69 3 838.00 5.04 242 66 113 47 357.56 0.73 243 66 116 50 356.74 0.65 244 66 157 91 354.42 0.39 245 67 116 49 357.37 0.72 246 67 147 80 354.26 0.32 247 67 155 88 356.72 0.72 248 67 157 90 354.43 0.39 249 67 161 94 278.94 1.25 250 67 162 95 278.41 1.14 251 68 81 13 361.72 0.59 252 68 85 17 361.48 0.58 253 68 161 93 278.61 1.08 254 68 162 94 278.43 1.12 255 68 163 95 277.82 0.84 256 68 166 98 268.86 1.32 257 69 85 16 361.27 0.48 258 69 113 44 357.78 0.60 259 69 115 46 356.96 0.60 260 69 116 47 356.87 0.60 261 69 157 88 354.32 0.35 262 69 161 92 278.56 1.09 263 70 77 7 361.97 0.72 264 70 113 43 357.49 0.73 265 70 114 44 357.54 0.65 266 70 115 45 356.98 0.59 267 71 77 6 401.93 19.25 268 71 78 7 361.84 0.68 269 71 81 10 361.71 0.70 270 71 166 95 268.79 1.33 271 71 169 98 268.70 1.36 272 72 76 4 558.15 13.45 273 72 77 5 361.96 0.79 274 73 76 3 850.91 1.81 275 73 78 5 457.37 7.77 276 77 82 5 812.57 7.65 277 77 115 38 364.94 1.43 278 78 81 3 852.73 0.86 279 78 82 4 820.41 7.79 280 78 169 91 268.62 1.32 281 79 82 3 832.09 1.95 282 79 83 4 830.72 2.64 283 80 83 3 839.35 1.08 284 80 84 4 826.66 0.60 285 80 169 89 268.57 1.28 286 81 84 3 842.69 0.67 287 81 85 4 826.65 0.59 288 81 160 79 351.57 0.03 289 81 165 84 276.26 0.01 290 81 166 85 268.79 1.33 291 81 169 88 267.99 1.01 292 82 85 3 837.41 3.31 293 82 86 4 822.99 0.53 294 82 115 33 366.61 1.26 295 83 86 3 833.33 1.94 296 83 87 4 825.68 1.88 297 84 87 3 850.83 1.94 298 84 160 76 353.54 0.35 299 85 89 4 825.38 2.04 300 85 115 30 405.44 17.67 301 85 158 73 364.80 1.38 302 85 160 75 353.25 0.24 303 85 161 76 347.11 1.43 304 86 89 3 821.05 2.85 305 86 115 29 413.23 20.66 306 87 160 73 365.66 0.95 307 88 142 54 385.54 6.19 308 88 143 55 385.54 6.19 309 89 94 5 832.03 4.77 310 89 114 25 497.09 31.52 311 89 115 26 496.99 31.52 312 89 117 28 496.91 31.46 313 89 144 55 395.96 11.39 314 89 156 67 428.43 0.56 315 89 157 68 418.38 0.20 316 89 158 69 395.96 11.39 317 90 93 3 864.53 1.75 318 90 94 4 832.03 4.77 319 90 142 52 427.85 0.75 320 91 94 3 841.03 0.27 321 91 95 4 825.54 1.53 322 91 112 21 497.31 31.42 323 91 113 22 497.29 31.46 324 91 114 23 497.23 31.46 325 91 116 25 497.10 31.52 326 91 117 26 497.03 31.52 327 92 95 3 852.44 1.64 328 92 96 4 831.25 1.02 329 93 96 3 841.96 0.72 330 93 97 4 831.25 1.02 331 93 142 49 431.76 1.12 332 94 97 3 842.16 0.62 333 94 98 4 825.73 1.74 334 94 117 23 497.30 31.43 335 94 137 43 432.23 1.04 336 94 142 48 431.76 1.12 337 94 144 50 431.74 1.13 338 94 156 62 431.77 1.12 339 95 98 3 842.16 0.62 340 95 99 4 840.52 0.18 341 95 117 22 497.33 31.44 342 95 119 24 497.58 31.29 343 96 99 3 858.98 7.19 344 96 100 4 840.55 0.18 345 97 100 3 865.58 3.90 346 97 101 4 863.76 4.79 347 97 133 36 432.70 1.21 348 97 136 39 432.70 1.21 349 97 137 40 432.70 1.21 350 98 101 3 868.15 2.60 351 98 104 6 497.72 31.21 352 98 119 21 497.76 31.25 353 98 133 35 432.70 1.22 354 98 156 58 432.55 1.19 355 99 119 20 497.77 31.28 356 101 104 3 844.62 0.01 357 101 129 28 462.24 15.16 358 101 132 31 455.55 11.81 359 101 133 32 438.97 3.61 360 101 136 35 451.11 9.63 361 103 120 17 527.40 16.49 362 103 121 18 527.07 16.57 363 103 122 19 527.01 16.54 364 103 125 22 501.30 3.70 365 103 129 26 462.81 15.42 366 104 119 15 527.24 16.70 367 104 120 16 527.39 16.48 368 104 121 17 527.12 16.59 369 104 133 29 462.77 15.49 370 104 154 50 462.49 15.68 371 105 119 14 527.54 16.55 372 105 120 15 527.40 16.48 373 106 118 12 527.56 16.56 374 106 119 13 527.54 16.55 375 107 111 4 803.19 4.05 376 107 112 5 768.18 21.56 377 107 118 11 527.57 16.57 378 107 119 12 527.57 16.55 379 107 120 13 527.61 16.54 380 107 153 46 462.82 15.86 381 107 154 47 462.80 15.86 382 108 111 3 840.85 3.66 383 108 112 4 822.12 5.71 384 109 112 3 828.27 2.63 385 111 116 5 552.62 4.28 386 111 118 7 528.37 16.30 387 112 116 4 537.03 12.07 388 112 117 5 528.47 16.34 389 112 118 6 528.38 16.30 390 113 116 3 811.08 0.01 391 115 157 42 462.64 16.56 392 115 158 43 462.62 16.55 393 116 155 39 462.82 16.54 394 116 156 40 462.72 16.59 395 116 157 41 462.68 16.57 396 117 155 38 463.06 16.43 397 117 156 39 462.72 16.59 398 118 154 36 463.12 16.43 399 118 155 37 463.09 16.43 400 119 153 34 463.59 16.24 401 119 154 35 463.17 16.43 402 119 156 37 463.16 16.43 403 120 152 32 463.75 16.16 404 120 153 33 463.59 16.24 405 121 125 4 806.02 3.78 406 121 127 6 485.75 5.24 407 121 129 8 464.06 16.07 408 121 130 9 464.04 16.09 409 121 148 27 463.86 16.16 410 121 152 31 463.76 16.17 411 122 125 3 816.50 0.35 412 125 129 4 847.25 3.04 413 126 129 3 851.38 1.42 414 126 130 4 847.45 2.94 415 127 130 3 847.45 2.94 416 127 131 4 840.14 0.52 417 127 148 21 464.99 15.95 418 128 131 3 835.02 1.69 419 128 132 4 834.85 1.76 420 129 132 3 845.40 3.50 421 129 133 4 843.75 4.34 422 130 133 3 846.40 3.02 423 130 134 4 838.54 6.95 424 130 146 16 465.00 15.93 425 130 148 18 465.04 15.93 426 130 154 24 465.01 15.93 427 131 134 3 851.66 0.39 428 131 135 4 835.88 2.23 429 132 135 3 846.43 3.03 430 132 136 4 835.85 2.24 431 133 136 3 852.63 6.15 432 133 137 4 837.19 1.56 433 133 146 13 465.50 16.16 434 133 154 21 465.67 16.20 435 133 156 23 465.50 16.16 436 134 137 3 837.20 1.57 437 134 138 4 837.18 1.56 438 134 146 12 465.03 15.93 439 135 138 3 837.19 1.56 440 135 139 4 837.19 1.56 441 136 139 3 848.69 4.19 442 136 140 4 833.94 1.61 443 137 140 3 839.21 2.46 444 137 144 7 465.16 16.01 445 137 146 9 465.84 16.30 446 137 156 19 466.41 16.57 447 142 159 17 467.95 16.92 448 143 158 15 467.97 16.92 449 143 159 16 467.95 16.92 450 144 156 12 468.38 17.09 451 144 157 13 468.07 16.95 452 144 158 14 467.98 16.92 453 144 159 15 467.96 16.92 454 145 155 10 469.44 17.00 455 145 156 11 468.78 16.94 456 145 157 12 468.11 16.96 457 145 158 13 468.10 16.96 458 145 159 14 467.99 16.92 459 145 161 16 467.99 16.93 460 146 154 8 469.50 16.98 461 146 155 9 469.45 16.99 462 146 156 10 469.14 16.89 463 147 153 6 494.75 15.81 464 147 154 7 469.50 16.98 465 147 155 8 469.48 16.99 466 147 157 10 469.47 16.98 467 148 151 3 890.68 2.07 468 148 152 4 844.18 3.48 469 148 153 5 505.30 10.54 470 148 154 6 469.50 16.98 471 149 152 3 877.27 2.39 472 149 153 4 677.62 57.48 473 157 161 4 774.38 27.75 474 158 161 3 838.63 4.99 475 160 165 5 607.93 11.59 476 162 165 3 843.73 1.96 477 162 166 4 797.63 19.95 478 163 166 3 840.08 4.44 479 163 167 4 830.63 3.46 480 163 190 27 198.19 0.55 481 163 193 30 198.11 0.55 482 164 167 3 832.50 2.54 483 164 168 4 832.80 2.38 484 164 192 28 198.04 0.54 485 164 193 29 198.09 0.55 486 165 168 3 833.81 1.88 487 165 169 4 832.79 2.38 488 166 169 3 846.02 4.82 489 166 170 4 836.19 0.06 490 167 170 3 836.74 0.33 491 167 171 4 835.49 0.41 492 167 183 16 265.62 0.54 493 167 193 26 198.03 0.54 494 167 196 29 197.52 0.54 495 168 171 3 844.56 3.58 496 168 172 4 848.31 6.11 497 169 172 3 856.15 2.19 498 169 173 4 835.49 0.41 499 170 173 3 845.10 4.48 500 170 174 4 835.49 0.41 501 171 174 3 843.07 3.37 502 171 175 4 830.64 2.75 503 171 176 5 592.16 12.10 504 171 178 7 511.18 26.56 505 171 181 10 265.62 0.54 506 171 196 25 197.51 0.54 507 171 197 26 197.46 0.55 508 171 198 27 197.37 0.55 509 172 175 3 813.83 1.22 510 176 180 4 704.28 38.02 511 177 180 3 846.83 5.25 512 177 181 4 841.88 7.71 513 178 181 3 833.02 1.66 514 178 197 19 197.35 0.55 515 178 198 20 197.28 0.55 516 179 199 20 197.25 0.56 517 179 242 63 35.02 0.66 518 180 242 62 27.12 1.79 519 181 198 17 197.26 0.57 520 181 199 18 197.25 0.56 521 182 199 17 197.26 0.56 522 182 200 18 197.24 0.57 523 182 201 19 197.24 0.57 524 182 236 54 55.76 0.84 525 182 237 55 55.06 0.51 526 182 240 58 36.51 0.35 527 182 242 60 31.41 0.57 528 183 193 10 197.99 0.53 529 183 194 11 197.83 0.59 530 183 196 13 197.57 0.53 531 183 198 15 197.42 0.55 532 183 199 16 197.36 0.55 533 183 200 17 197.23 0.57 534 183 201 18 197.23 0.57 535 184 193 9 198.09 0.56 536 184 201 17 197.21 0.57 537 184 233 49 70.67 1.05 538 185 190 5 353.59 78.27 539 185 194 9 198.10 0.56 540 185 201 16 197.30 0.55 541 185 202 17 197.21 0.57 542 185 203 18 197.18 0.57 543 185 210 25 150.33 2.35 544 186 190 4 826.28 0.10 545 186 203 17 197.21 0.57 546 186 206 20 153.03 2.98 547 186 207 21 151.28 2.10 548 186 210 24 150.34 2.34 549 187 190 3 842.36 0.30 550 187 191 4 839.61 1.69 551 187 207 20 150.60 2.44 552 187 210 23 150.37 2.35 553 188 191 3 848.40 2.71 554 188 209 21 150.34 2.33 555 188 210 22 150.33 2.34 556 190 193 3 843.20 0.37 557 190 194 4 837.64 3.16 558 191 194 3 844.66 0.35 559 191 195 4 837.64 3.16 560 191 209 18 150.32 2.33 561 191 210 19 150.16 2.33 562 191 213 22 140.05 0.71 563 192 195 3 849.50 2.77 564 192 196 4 837.64 3.15 565 193 196 3 859.85 6.45 566 194 200 6 197.51 0.53 567 194 201 7 197.51 0.53 568 194 202 8 197.44 0.55 569 194 210 16 150.28 2.34 570 194 213 19 140.03 0.72 571 194 222 28 108.00 0.48 572 197 221 24 108.00 0.48 573 198 221 23 107.98 0.48 574 199 221 22 107.95 0.49 575 199 240 41 51.03 0.30 576 199 242 43 42.16 0.46 577 200 221 21 108.00 0.48 578 200 222 22 107.94 0.49 579 200 223 23 107.92 0.49 580 200 240 40 53.88 0.29 581 201 223 22 107.93 0.49 582 201 225 24 107.86 0.52 583 201 232 31 72.55 0.41 584 201 233 32 70.67 1.05 585 201 236 35 63.91 0.93 586 202 206 4 711.31 51.91 587 202 210 8 150.32 2.35 588 202 211 9 150.28 2.35 589 202 214 12 139.82 0.30 590 202 223 21 108.00 0.48 591 202 224 22 107.91 0.51 592 202 225 23 107.88 0.52 593 203 206 3 835.73 3.51 594 203 225 22 107.89 0.52 595 203 228 25 85.47 0.65 596 203 229 26 77.66 0.20 597 203 232 29 73.32 0.68 598 204 224 20 108.00 0.49 599 204 225 21 107.91 0.53 600 204 226 22 107.89 0.52 601 204 227 23 107.78 0.48 602 204 228 24 92.59 0.45 603 205 228 23 98.21 0.53 604 205 229 24 95.14 0.84 605 206 210 4 834.72 1.38 606 206 211 5 831.71 2.39 607 207 210 3 851.16 0.29 608 207 211 4 834.73 1.38 609 208 211 3 834.74 1.38 610 208 212 4 834.73 1.38 611 209 212 3 840.82 4.41 612 209 213 4 834.73 1.38 613 210 213 3 843.77 5.92 614 210 214 4 834.14 1.10 615 211 214 3 834.50 1.28 616 211 215 4 834.46 1.30 617 211 224 13 108.05 0.48 618 212 215 3 849.16 5.89 619 212 216 4 834.45 1.31 620 213 216 3 840.83 4.51 621 213 217 4 809.36 8.64 622 213 218 5 804.49 11.07 623 213 222 9 108.34 0.48 624 214 217 3 851.28 5.54 625 214 218 4 661.20 72.31 626 214 222 8 108.26 0.48 627 214 224 10 108.05 0.48 628 218 222 4 514.03 13.55 629 219 222 3 842.24 2.58 630 221 240 19 54.73 0.66 631 223 235 12 70.67 1.05 632 223 236 13 69.25 0.55 633 223 239 16 56.49 0.58 634 223 240 17 55.76 0.84 635 225 232 7 73.73 0.05 636 225 235 10 70.72 1.05 637 226 235 9 293.74 109.23 638 227 231 4 653.01 76.64 639 227 232 5 501.56 17.35 640 227 235 8 293.74 109.23 641 228 231 3 853.09 0.95 642 228 232 4 702.30 59.55 643 229 232 3 844.44 1.25 644 230 233 3 837.27 0.37 645 230 234 4 833.12 2.01 646 231 234 3 844.68 1.33 647 231 235 4 833.12 2.01 648 232 235 3 832.86 2.15 649 232 236 4 832.86 2.14 650 233 236 3 843.16 3.01 651 233 237 4 832.68 2.05 652 234 237 3 833.75 1.54 653 234 238 4 833.70 1.54 654 235 238 3 851.39 4.54 655 235 239 4 832.66 2.05 656 236 239 3 836.87 0.04 657 236 240 4 823.62 6.58 658 237 240 3 843.18 0.44 659 237 241 4 816.39 3.56 660 237 242 5 510.42 21.27 661 237 243 6 511.75 20.60 662 238 241 3 821.72 0.36 663 241 244 3 867.52 1.16