BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 57 60 3 819.69 1.35 2 57 61 4 809.05 1.20 3 58 61 3 826.31 3.06 4 58 62 4 805.21 0.72 5 59 62 3 811.92 2.64 6 59 63 4 811.91 2.65 7 60 63 3 818.71 0.74 8 60 64 4 810.16 3.52 9 61 64 3 818.68 0.73 10 61 65 4 810.16 3.52 11 62 65 3 818.69 0.73 12 62 66 4 818.66 0.74 13 63 66 3 817.32 1.42 14 63 67 4 807.47 2.21 15 64 67 3 820.95 1.97 16 64 68 4 810.03 3.48 17 65 68 3 821.00 1.94 18 65 69 4 807.49 2.24 19 66 69 3 810.25 3.43 20 66 70 4 819.92 1.40 21 67 70 3 819.93 1.41 22 67 71 4 817.38 2.67 23 67 272 210 60.88 0.48 24 67 274 212 57.59 1.15 25 68 71 3 817.38 2.67 26 68 72 4 817.38 2.67 27 68 158 95 313.28 80.61 28 69 72 3 817.47 2.64 29 69 73 4 817.38 2.67 30 69 267 203 80.08 0.56 31 70 73 3 828.25 3.44 32 70 74 4 814.18 12.02 33 70 267 202 80.08 0.56 34 70 274 209 80.05 0.58 35 70 277 212 80.04 0.58 36 71 74 3 827.53 5.34 37 71 75 4 824.98 6.62 38 71 161 95 325.93 85.25 39 71 274 208 83.33 2.16 40 71 276 210 83.31 2.16 41 71 277 211 80.11 0.57 42 72 75 3 829.09 4.57 43 72 76 4 819.94 9.13 44 72 92 25 335.94 85.14 45 72 93 26 331.50 83.64 46 72 94 27 332.07 83.36 47 72 161 94 328.44 84.44 48 73 76 3 827.56 5.33 49 73 77 4 827.56 5.32 50 73 264 196 100.25 0.12 51 73 266 198 100.59 0.23 52 73 267 199 100.46 0.21 53 74 77 3 819.36 1.22 54 74 78 4 809.82 3.56 55 74 262 193 100.16 0.11 56 74 263 194 100.41 0.21 57 74 277 208 100.45 0.21 58 74 280 211 100.43 0.20 59 75 78 3 809.82 3.56 60 75 79 4 809.82 3.56 61 75 93 23 341.69 82.58 62 75 161 91 332.09 83.36 63 75 164 94 331.52 83.64 64 75 165 95 331.54 83.64 65 75 168 98 330.92 83.43 66 75 276 206 101.14 0.16 67 75 280 210 100.73 0.29 68 76 79 3 820.88 0.46 69 76 80 4 809.82 3.56 70 76 90 19 342.31 82.59 71 76 93 22 341.68 82.59 72 77 80 3 818.38 0.78 73 77 81 4 816.88 0.03 74 77 262 190 100.89 0.17 75 77 264 192 101.19 0.13 76 78 81 3 827.49 6.27 77 78 82 4 815.80 0.52 78 78 106 33 342.67 82.59 79 78 110 37 342.63 82.61 80 78 164 91 342.12 82.62 81 78 167 94 341.67 82.84 82 78 168 95 339.11 81.87 83 78 172 99 320.79 82.84 84 78 262 189 101.10 0.12 85 78 280 207 101.35 0.05 86 79 82 3 820.38 2.81 87 79 83 4 815.85 0.47 88 79 90 16 343.02 82.69 89 79 93 19 342.85 82.67 90 79 106 32 342.36 82.75 91 79 164 90 342.17 82.64 92 80 83 3 815.88 0.48 93 80 84 4 815.86 0.47 94 80 89 14 343.61 82.47 95 80 90 15 343.00 82.70 96 81 84 3 828.90 2.31 97 81 85 4 818.39 0.77 98 81 260 184 100.97 0.18 99 82 85 3 821.22 0.65 100 82 85 4 818.34 0.80 101 82 106 29 343.02 82.73 102 82 109 32 343.06 82.75 103 82 110 33 343.00 82.73 104 83 85 3 827.25 3.66 105 83 86 8 344.29 82.48 106 83 87 9 344.21 82.44 107 83 88 10 344.21 82.45 108 83 90 12 344.20 82.45 109 83 102 24 343.76 82.42 110 83 105 27 343.10 82.75 111 83 106 28 343.06 82.76 112 84 87 8 344.20 82.44 113 85 109 29 343.57 82.64 114 85 86 5 483.97 81.64 115 85 87 6 344.31 82.43 116 85 105 24 344.13 82.42 117 85 108 27 344.08 82.39 118 85 109 28 344.08 82.39 119 85 108 26 344.18 82.41 120 85 109 27 344.16 82.41 121 85 112 30 344.14 82.42 122 85 86 3 824.63 0.89 123 85 87 4 794.62 2.63 124 85 117 34 344.36 82.45 125 85 87 3 824.63 0.89 126 85 88 3 847.57 0.60 127 86 105 19 344.22 82.40 128 88 101 13 344.57 82.44 129 88 102 14 344.43 82.47 130 90 93 3 841.57 5.67 131 90 96 6 409.52 51.25 132 90 97 7 466.50 75.62 133 90 102 12 345.94 83.03 134 91 96 5 718.31 14.94 135 92 95 3 810.86 1.23 136 92 96 4 752.35 1.28 137 93 97 4 802.66 0.86 138 93 160 67 355.60 84.16 139 93 161 68 355.60 84.17 140 93 164 71 352.55 85.62 141 94 98 4 805.94 1.81 142 94 155 61 380.83 71.66 143 94 157 63 380.50 71.78 144 94 160 66 371.23 76.42 145 94 161 67 371.24 76.42 146 95 98 3 834.16 0.06 147 96 102 6 431.25 61.94 148 97 100 3 807.13 1.22 149 97 102 5 463.35 74.09 150 97 103 6 431.24 61.94 151 97 152 55 429.94 81.48 152 97 160 63 420.18 82.43 153 97 164 67 402.29 76.41 154 98 130 32 435.56 81.64 155 98 152 54 429.94 81.49 156 98 153 55 429.95 81.49 157 98 154 56 433.86 81.79 158 98 155 57 429.90 81.50 159 99 126 27 436.53 82.02 160 99 129 30 436.78 81.95 161 100 103 3 820.18 3.25 162 100 104 4 793.88 4.45 163 100 129 29 437.47 82.09 164 100 130 30 436.48 82.00 165 101 104 3 814.89 3.81 166 101 105 4 802.86 1.73 167 101 117 16 438.52 82.23 168 101 118 17 438.53 82.23 169 101 129 28 437.89 82.12 170 102 105 3 809.99 1.83 171 102 106 4 802.85 1.72 172 103 106 3 807.80 4.19 173 103 107 4 814.05 3.85 174 103 133 30 438.81 82.29 175 103 152 49 437.91 82.14 176 103 163 60 437.21 82.14 177 103 167 64 437.01 82.11 178 104 107 3 820.30 0.73 179 104 108 4 799.41 3.44 180 104 115 11 439.10 82.38 181 104 116 12 439.12 82.38 182 104 117 13 439.01 82.34 183 104 129 25 438.80 82.25 184 104 132 28 438.84 82.25 185 104 133 29 438.84 82.29 186 105 108 3 802.87 1.74 187 105 109 4 798.71 3.10 188 105 117 12 442.02 80.83 189 106 109 3 820.23 0.77 190 106 110 4 798.70 3.10 191 106 164 58 437.22 82.14 192 106 167 61 437.13 82.17 193 107 110 3 797.68 2.59 194 107 111 4 791.86 0.27 195 107 115 8 458.51 76.36 196 107 133 26 439.14 82.23 197 107 136 29 439.25 82.17 198 107 137 30 439.24 82.17 199 107 167 60 437.14 82.17 200 107 171 64 434.92 81.62 201 108 111 3 807.51 2.33 202 108 112 4 710.80 32.26 203 108 113 5 516.65 84.98 204 108 114 6 458.53 76.36 205 108 115 7 458.53 76.36 206 108 117 9 458.52 76.36 207 110 167 57 437.18 82.17 208 110 171 61 434.90 81.64 209 111 136 25 441.50 83.20 210 111 140 29 441.88 82.81 211 111 171 60 435.38 81.85 212 113 136 23 443.79 82.44 213 115 132 17 444.23 82.41 214 115 136 21 444.05 82.39 215 116 132 16 444.52 82.53 216 117 129 12 444.59 82.55 217 117 132 15 444.57 82.55 218 118 125 7 444.70 82.50 219 118 128 10 444.69 82.50 220 118 129 11 444.60 82.55 221 118 132 14 444.61 82.55 222 120 125 5 738.78 5.84 223 121 125 4 791.08 1.76 224 121 126 5 783.68 1.95 225 122 125 3 814.06 2.88 226 122 126 4 807.22 6.29 227 123 126 3 813.44 3.19 228 123 127 4 804.65 3.99 229 123 150 27 444.60 82.69 230 123 153 30 444.47 82.72 231 123 154 31 444.47 82.72 232 124 127 3 819.36 0.23 233 124 128 4 804.66 4.00 234 125 128 3 819.36 0.23 235 125 129 4 804.31 4.17 236 126 129 3 815.77 1.55 237 126 130 4 815.75 1.56 238 127 130 3 815.75 1.57 239 127 131 4 813.75 2.56 240 127 149 22 444.59 82.70 241 127 150 23 444.60 82.69 242 127 153 26 444.43 82.75 243 128 131 3 813.76 2.56 244 128 132 4 813.80 2.54 245 129 132 3 819.06 0.10 246 129 133 4 813.79 2.54 247 130 133 3 822.62 1.69 248 130 134 4 802.35 3.17 249 130 149 19 444.61 82.70 250 130 152 22 444.47 82.75 251 131 134 3 802.34 3.17 252 131 135 4 789.17 3.18 253 131 149 18 444.61 82.70 254 132 135 3 810.49 2.59 255 132 136 4 802.77 2.93 256 133 136 3 815.86 1.57 257 133 137 4 795.43 0.74 258 134 137 3 823.52 2.26 259 134 138 4 803.75 3.42 260 134 144 10 445.22 82.66 261 134 147 13 444.78 82.72 262 134 149 15 444.66 82.73 263 134 163 29 437.65 82.20 264 135 138 3 810.99 2.81 265 135 139 4 795.27 0.82 266 136 139 3 817.73 0.56 267 136 140 4 792.59 2.15 268 137 140 3 813.01 1.80 269 137 141 4 794.35 5.51 270 137 142 5 716.90 2.09 271 137 144 7 498.56 73.01 272 137 163 26 437.90 82.23 273 137 167 30 437.38 82.09 274 137 171 34 436.64 82.11 275 138 141 3 785.24 4.26 276 138 142 4 772.21 3.46 277 138 144 6 688.70 10.65 278 140 170 30 436.89 82.15 279 140 171 31 436.76 82.17 280 142 166 24 437.70 82.17 281 142 170 28 436.88 82.08 282 144 147 3 843.26 4.04 283 144 163 19 438.19 82.17 284 144 166 22 438.09 82.19 285 146 159 13 438.39 82.20 286 147 151 4 801.71 3.84 287 147 152 5 798.98 3.62 288 147 159 12 438.37 82.20 289 147 163 16 438.26 82.16 290 148 151 3 815.66 0.30 291 148 152 4 808.39 0.51 292 149 152 3 818.45 0.47 293 149 153 4 808.39 0.51 294 150 153 3 811.78 2.20 295 150 154 4 798.28 2.17 296 151 154 3 814.06 2.54 297 151 155 4 808.37 0.49 298 151 156 5 762.78 14.38 299 151 159 8 451.94 75.45 300 152 155 3 808.37 0.50 301 152 159 7 457.71 78.34 302 152 160 8 438.34 82.22 303 152 163 11 438.14 82.12 304 156 159 3 822.55 5.04 305 156 160 4 784.65 9.11 306 157 160 3 810.19 1.15 307 157 161 4 797.18 0.08 308 158 161 3 813.66 2.73 309 158 162 4 797.14 0.10 310 159 162 3 800.72 1.69 311 159 163 4 797.14 0.10 312 160 163 3 806.34 1.12 313 160 164 4 793.41 7.58 314 161 164 3 806.34 1.12 315 161 165 4 793.41 7.58 316 162 165 3 793.98 0.94 317 162 166 4 793.88 0.89 318 163 166 3 817.96 0.04 319 163 167 4 793.88 0.89 320 164 167 3 797.68 0.24 321 164 168 4 791.18 2.25 322 165 168 3 801.59 2.19 323 165 169 4 787.34 4.17 324 165 173 8 731.43 10.05 325 165 276 111 101.60 0.06 326 165 279 114 101.61 0.06 327 165 280 115 101.50 0.01 328 166 169 3 806.97 1.30 329 166 170 4 786.89 3.96 330 167 170 3 803.23 3.00 331 167 171 4 786.88 3.97 332 168 171 3 822.66 2.12 333 168 172 4 781.95 1.51 334 168 173 5 781.93 1.50 335 168 280 112 101.59 0.04 336 169 173 4 768.85 3.64 337 169 174 5 768.85 3.64 338 169 187 18 103.77 0.40 339 170 174 4 768.82 3.65 340 172 280 108 103.14 0.01 341 172 283 111 102.56 0.01 342 172 284 112 102.05 0.24 343 172 287 115 96.98 2.31 344 173 176 3 848.52 2.41 345 173 187 14 103.90 0.34 346 173 191 18 103.84 0.35 347 173 279 106 104.00 0.29 348 173 283 110 102.61 0.03 349 175 286 111 103.91 0.33 350 176 181 5 803.53 2.80 351 176 190 14 105.06 0.47 352 176 191 15 105.06 0.47 353 176 194 18 105.05 0.47 354 176 286 110 103.72 0.23 355 177 181 4 803.53 2.80 356 177 286 109 105.04 0.47 357 177 292 115 60.46 1.92 358 178 181 3 821.82 2.56 359 178 190 12 105.31 0.55 360 178 193 15 105.33 0.54 361 178 194 16 105.06 0.47 362 178 197 19 105.09 0.46 363 178 292 114 57.66 0.52 364 179 197 18 105.16 0.48 365 179 293 114 56.70 1.00 366 180 193 13 105.50 0.57 367 180 197 17 105.41 0.59 368 181 185 4 809.69 0.67 369 181 190 9 105.48 0.57 370 181 193 12 105.64 0.48 371 182 185 3 821.48 2.74 372 185 189 4 794.92 1.56 373 185 190 5 791.95 0.08 374 185 193 8 707.32 5.29 375 186 189 3 802.88 5.50 376 186 190 4 794.96 1.55 377 187 190 3 804.93 6.51 378 187 191 4 801.44 4.77 379 188 191 3 817.56 1.79 380 188 192 4 801.45 4.77 381 188 210 22 326.48 84.66 382 188 275 87 332.11 81.84 383 188 279 91 325.13 84.09 384 189 192 3 816.09 2.53 385 189 193 4 801.45 4.77 386 190 193 3 817.56 1.79 387 190 194 4 802.93 5.51 388 191 194 3 802.53 5.70 389 191 195 4 797.58 6.72 390 191 210 19 338.31 81.32 391 191 279 88 334.13 80.84 392 191 282 91 325.57 84.21 393 191 283 92 325.57 84.21 394 191 286 95 325.30 84.08 395 192 195 3 800.80 5.11 396 192 196 4 797.57 6.71 397 192 207 15 338.69 81.36 398 192 209 17 338.50 81.39 399 192 210 18 338.48 81.41 400 193 196 3 807.87 10.56 401 193 197 4 785.12 0.58 402 194 197 3 789.52 2.78 403 194 198 4 791.73 0.90 404 194 223 29 338.47 81.31 405 194 282 88 338.25 81.27 406 194 286 92 336.38 81.36 407 194 289 95 336.11 81.35 408 194 293 99 21.09 0.30 409 195 198 3 818.55 2.27 410 195 199 4 794.22 0.44 411 195 207 12 338.75 81.36 412 195 214 19 338.73 81.36 413 195 223 28 338.44 81.30 414 195 282 87 338.26 81.27 415 196 199 3 827.86 6.92 416 196 200 4 793.48 0.07 417 196 207 11 338.75 81.36 418 197 200 3 807.55 4.28 419 197 226 29 338.62 81.36 420 197 293 96 25.30 2.35 421 198 202 4 843.96 0.53 422 198 223 25 338.58 81.37 423 198 226 28 338.58 81.37 424 198 289 91 336.25 81.34 425 198 293 95 29.43 4.42 426 199 202 3 825.51 1.87 427 199 205 6 430.65 35.63 428 199 219 20 338.67 81.37 429 199 222 23 338.62 81.36 430 199 223 24 338.62 81.36 431 202 205 3 850.07 2.27 432 202 222 20 339.01 81.42 433 202 225 23 339.01 81.42 434 202 226 24 339.01 81.42 435 202 234 32 339.06 81.42 436 202 238 36 340.53 81.83 437 203 238 35 340.54 81.84 438 205 218 13 340.58 81.86 439 205 219 14 340.53 81.84 440 205 222 17 340.53 81.84 441 205 234 29 340.57 81.86 442 206 219 13 340.70 81.81 443 207 213 6 434.61 35.13 444 207 214 7 434.61 35.13 445 207 219 12 340.84 81.73 446 208 213 5 770.29 6.74 447 209 212 3 872.98 4.54 448 209 213 4 779.10 2.35 449 209 214 5 788.07 2.13 450 210 214 4 808.59 0.69 451 210 275 65 393.73 82.52 452 210 278 68 376.32 80.95 453 210 279 69 376.29 80.97 454 210 282 72 368.70 77.14 455 211 214 3 831.21 1.19 456 211 215 4 809.47 0.25 457 211 272 61 422.04 86.38 458 211 273 62 421.08 86.33 459 211 275 64 415.00 84.25 460 212 215 3 831.22 1.19 461 212 271 59 426.22 84.98 462 212 272 60 422.04 86.38 463 212 273 61 422.05 86.38 464 213 219 6 716.99 19.89 465 214 217 3 826.74 6.45 466 214 219 5 716.99 19.89 467 214 220 6 717.00 19.89 468 214 223 9 717.00 19.88 469 214 268 54 430.38 84.67 470 214 278 64 422.07 86.36 471 214 282 68 422.00 86.39 472 215 242 27 512.78 88.61 473 215 246 31 512.75 88.61 474 215 268 53 430.50 84.73 475 215 269 54 430.69 84.69 476 215 270 55 431.56 84.89 477 215 271 56 431.82 85.03 478 216 242 26 512.77 88.61 479 216 245 29 512.78 88.61 480 217 220 3 811.74 0.97 481 217 221 4 802.82 2.07 482 217 245 28 512.79 88.61 483 217 246 29 512.77 88.62 484 218 221 3 813.52 2.40 485 218 222 4 802.14 1.73 486 218 234 16 513.82 88.29 487 218 237 19 513.69 88.31 488 218 238 20 513.72 88.32 489 219 222 3 815.40 2.58 490 219 223 4 793.46 3.44 491 220 223 3 824.66 2.05 492 220 224 4 799.51 0.41 493 220 246 26 513.68 88.34 494 220 249 29 513.38 88.39 495 220 250 30 513.38 88.39 496 220 268 48 432.80 85.14 497 220 278 58 431.92 85.04 498 220 281 61 431.88 85.05 499 220 282 62 431.74 85.09 500 220 285 65 431.51 84.99 501 221 224 3 806.15 3.73 502 221 225 4 796.11 1.29 503 221 232 11 513.90 88.29 504 221 234 13 513.87 88.31 505 221 237 16 513.84 88.30 506 221 245 24 513.70 88.33 507 221 248 27 513.64 88.33 508 221 249 28 513.40 88.37 509 222 225 3 800.54 0.92 510 222 226 4 787.73 2.87 511 222 234 12 513.86 88.31 512 223 226 3 792.60 5.29 513 223 227 4 806.87 2.25 514 223 285 62 431.50 84.98 515 223 289 66 425.91 84.86 516 224 227 3 806.88 2.25 517 224 228 4 786.79 2.40 518 224 232 8 513.96 88.28 519 224 252 28 513.38 88.26 520 224 253 29 513.19 88.29 521 224 257 33 512.42 88.27 522 224 285 61 431.52 84.99 523 224 289 65 426.22 85.00 524 225 228 3 800.15 1.09 525 225 229 4 700.78 26.86 526 225 232 7 513.95 88.29 527 225 234 9 513.92 88.30 528 227 257 30 512.79 88.41 529 227 289 62 426.01 84.81 530 227 290 63 425.96 84.83 531 228 231 3 822.10 0.56 532 228 232 4 809.32 5.83 533 228 257 29 512.77 88.39 534 229 232 3 826.70 2.86 535 231 252 21 513.48 88.23 536 231 256 25 512.70 88.36 537 232 237 5 801.97 1.35 538 232 248 16 513.66 88.30 539 232 252 20 513.49 88.25 540 233 236 3 820.62 0.35 541 233 237 4 806.13 3.43 542 234 237 3 817.16 2.08 543 234 238 4 814.21 0.60 544 235 238 3 831.39 0.97 545 236 239 3 818.80 1.24 546 236 240 4 810.03 2.68 547 237 240 3 818.81 1.24 548 237 245 8 513.75 88.35 549 237 248 11 513.75 88.35 550 240 244 4 787.01 4.48 551 240 245 5 777.45 9.27 552 240 248 8 753.26 2.60 553 241 244 3 797.99 0.40 554 241 245 4 787.03 4.48 555 242 245 3 831.50 4.16 556 242 246 4 797.27 0.64 557 242 269 27 432.68 85.11 558 243 246 3 797.28 0.64 559 243 247 4 797.27 0.64 560 243 265 22 432.87 85.19 561 243 266 23 432.88 85.19 562 243 269 26 432.69 85.12 563 244 247 3 805.59 0.56 564 244 248 4 798.27 0.15 565 245 248 3 803.61 2.50 566 245 249 4 798.27 0.15 567 246 249 3 810.96 1.18 568 246 250 4 798.28 0.15 569 246 265 19 432.87 85.19 570 246 268 22 432.86 85.18 571 246 269 23 432.81 85.15 572 247 250 3 809.27 2.02 573 247 251 4 792.74 4.71 574 247 265 18 433.20 85.02 575 248 251 3 800.50 0.84 576 248 252 4 792.79 4.69 577 249 252 3 808.95 2.81 578 249 253 4 786.29 5.88 579 250 253 3 808.38 3.12 580 250 254 4 806.33 4.13 581 250 261 11 433.68 85.06 582 250 263 13 433.47 85.08 583 250 264 14 433.44 85.08 584 250 265 15 433.37 85.08 585 250 268 18 433.37 85.07 586 250 281 31 432.44 85.14 587 251 254 3 812.96 0.83 588 251 255 4 798.32 0.27 589 251 261 10 434.27 84.82 590 252 255 3 809.99 2.31 591 252 256 4 778.24 9.77 592 252 257 5 741.68 8.51 593 253 257 4 730.43 3.35 594 253 258 5 674.26 23.82 595 253 259 6 450.97 77.71 596 253 281 28 432.57 85.09 597 253 285 32 431.75 85.05 598 254 258 4 503.63 83.86 599 254 259 5 455.73 80.00 600 254 261 7 434.34 84.83 601 257 288 31 429.46 84.29 602 257 289 32 429.46 84.29 603 259 281 22 432.70 85.06 604 259 284 25 432.12 85.14 605 259 285 26 431.88 85.00 606 259 288 29 431.19 85.11 607 260 281 21 432.74 85.05 608 262 280 18 432.78 85.06 609 262 281 19 432.64 85.08 610 263 268 5 747.88 3.14 611 263 277 14 432.81 85.06 612 263 278 15 432.80 85.06 613 263 281 18 432.61 85.09 614 264 267 3 800.12 2.93 615 264 268 4 800.12 2.93 616 265 268 3 806.80 2.37 617 265 269 4 753.89 0.17 618 267 270 3 863.93 0.51 619 268 273 5 433.27 84.84 620 268 278 10 432.70 84.98 621 270 273 3 816.01 5.98 622 273 278 5 748.23 3.06 623 274 277 3 812.48 5.54 624 274 278 4 791.80 2.40 625 275 278 3 825.26 0.85 626 275 279 4 791.80 2.40 627 276 279 3 795.16 3.39 628 276 280 4 771.62 12.48 629 277 280 3 798.50 2.58 630 277 281 4 795.18 0.70 631 278 281 3 809.31 1.65 632 278 282 4 789.89 2.77 633 279 282 3 807.25 2.68 634 279 283 4 790.63 2.40 635 280 283 3 808.16 2.23 636 280 284 4 795.12 3.40 637 281 284 3 809.28 0.46 638 281 285 4 807.94 2.10 639 282 285 3 807.94 2.10 640 282 286 4 807.94 2.10 641 283 286 3 807.92 2.10 642 283 287 4 807.82 2.16 643 284 287 3 807.82 2.16 644 284 288 4 807.82 2.16 645 285 288 3 814.38 1.12 646 285 289 4 807.81 2.16 647 286 289 3 807.86 2.12