BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 22 51 29 23.77 1.11 2 22 52 30 25.55 0.92 3 22 53 31 27.48 0.44 4 22 56 34 36.52 0.54 5 22 59 37 33.69 0.57 6 22 164 141 26.42 1.00 7 23 51 28 26.67 0.84 8 23 52 29 26.67 0.84 9 23 53 30 27.88 0.55 10 23 56 33 36.63 0.53 11 24 53 29 34.20 0.24 12 24 54 30 36.27 0.48 13 24 55 31 36.63 0.54 14 24 56 32 37.21 0.24 15 25 28 3 842.68 3.42 16 25 53 28 36.36 0.45 17 25 54 29 36.61 0.55 18 26 29 3 838.10 6.11 19 27 30 3 843.58 3.37 20 28 31 3 844.34 2.98 21 28 32 4 827.33 0.72 22 28 43 15 42.48 0.34 23 28 52 24 42.49 0.34 24 28 53 25 42.55 0.31 25 28 54 26 42.55 0.31 26 28 257 228 38.55 0.43 27 28 261 232 38.72 0.35 28 29 32 3 813.15 2.42 29 29 33 4 802.71 7.63 30 29 54 25 42.69 0.27 31 30 33 3 818.18 0.04 32 30 34 4 801.59 3.98 33 31 253 221 44.24 1.12 34 31 256 224 42.61 0.32 35 31 257 225 42.61 0.32 36 32 35 3 829.18 3.31 37 32 36 4 722.81 20.73 38 32 37 5 606.39 32.34 39 32 39 7 584.34 21.32 40 32 40 8 559.82 33.57 41 32 41 9 523.66 24.54 42 32 54 22 81.07 14.48 43 32 237 204 53.17 0.51 44 32 253 220 46.68 0.07 45 32 257 224 46.69 0.09 46 33 36 3 745.09 31.87 47 35 249 213 66.45 2.32 48 37 239 201 113.88 1.61 49 37 249 211 100.05 0.15 50 37 253 215 90.20 0.23 51 38 58 20 138.28 1.76 52 38 239 200 114.06 1.70 53 38 240 201 114.05 1.70 54 39 54 15 527.10 24.00 55 39 58 19 146.39 2.29 56 39 238 198 115.12 2.22 57 39 239 199 114.06 1.70 58 40 53 13 531.55 26.16 59 40 54 14 527.08 24.02 60 40 55 15 365.37 97.16 61 40 58 18 154.48 1.57 62 40 59 19 147.59 1.72 63 40 163 122 138.28 1.77 64 40 237 196 130.97 1.79 65 40 238 197 125.86 0.53 66 41 53 12 531.55 26.16 67 41 54 13 531.52 26.15 68 41 235 193 171.13 0.04 69 41 236 194 170.38 0.09 70 41 237 195 138.24 1.78 71 41 254 212 105.33 1.19 72 41 257 215 100.31 0.03 73 41 258 216 100.30 0.03 74 42 51 9 862.84 1.01 75 42 52 10 704.04 15.79 76 42 53 11 531.57 26.16 77 42 59 17 365.24 97.09 78 42 163 120 171.12 0.05 79 42 164 121 171.15 0.04 80 42 167 124 171.18 0.05 81 42 235 192 171.18 0.05 82 42 236 193 171.09 0.06 83 43 51 8 972.48 5.56 84 43 52 9 821.64 2.21 85 43 233 189 180.36 1.82 86 43 234 190 174.81 0.67 87 43 235 191 171.19 0.05 88 43 258 214 100.56 0.09 89 43 261 217 94.10 1.16 90 43 263 219 92.14 1.14 91 44 167 122 182.78 2.14 92 44 233 188 189.48 0.04 93 44 234 189 176.40 0.12 94 45 231 185 197.41 0.58 95 45 232 186 197.35 0.61 96 45 233 187 196.25 0.82 97 46 231 184 197.41 0.58 98 46 232 185 197.41 0.58 99 46 234 187 197.37 0.61 100 47 171 123 197.47 0.61 101 47 234 186 197.41 0.58 102 48 167 118 197.47 0.61 103 48 168 119 197.47 0.61 104 48 171 122 197.49 0.60 105 53 56 3 832.86 2.10 106 53 59 6 651.36 10.76 107 55 58 3 820.12 1.47 108 55 59 4 800.39 3.67 109 56 59 3 813.26 7.23 110 57 60 3 841.25 1.24 111 57 61 4 800.62 3.56 112 58 61 3 803.26 2.23 113 58 63 5 815.80 6.68 114 58 238 179 197.64 0.56 115 58 239 180 197.61 0.56 116 58 240 181 197.60 0.55 117 59 162 102 349.74 23.27 118 59 163 103 323.81 26.84 119 59 164 104 315.43 31.04 120 60 162 101 354.42 25.61 121 60 164 103 335.51 30.39 122 61 162 100 371.66 19.66 123 62 143 80 410.33 27.48 124 62 160 97 410.79 26.49 125 62 161 98 382.62 25.14 126 62 162 99 382.62 25.14 127 63 160 96 411.69 26.94 128 63 161 97 411.30 26.75 129 63 219 155 260.02 24.55 130 63 221 157 273.39 17.81 131 63 238 174 224.64 0.34 132 64 153 88 436.25 22.87 133 64 154 89 435.75 23.07 134 64 155 90 436.32 22.85 135 64 156 91 436.24 22.87 136 64 157 92 436.19 22.85 137 64 159 94 416.43 29.04 138 64 160 95 413.18 27.46 139 64 209 144 265.75 23.07 140 64 219 154 261.06 25.06 141 64 221 156 274.73 18.20 142 65 68 3 821.40 7.62 143 65 69 4 698.23 22.82 144 65 141 75 453.22 27.19 145 65 154 88 436.31 22.84 146 65 159 93 433.79 22.84 147 65 160 94 420.39 28.62 148 65 161 95 418.00 29.81 149 65 221 155 274.77 18.21 150 66 69 3 833.61 1.52 151 66 154 87 436.37 22.84 152 66 155 88 436.38 22.84 153 66 207 140 274.82 18.53 154 66 208 141 274.79 18.57 155 66 209 142 265.79 23.09 156 67 115 47 438.79 23.50 157 67 205 137 276.94 17.48 158 67 206 138 276.64 17.64 159 67 207 139 274.85 18.54 160 68 72 3 884.05 0.29 161 68 166 97 420.90 29.53 162 68 205 136 276.94 17.48 163 68 206 137 276.51 17.79 164 68 207 138 276.19 19.21 165 68 221 152 277.78 18.44 166 68 223 154 276.94 17.48 167 69 73 3 820.82 3.10 168 69 132 62 476.60 22.91 169 69 136 66 476.82 23.00 170 69 141 71 476.85 22.96 171 69 153 83 437.36 22.84 172 69 154 84 437.36 22.84 173 69 159 89 437.14 22.90 174 71 74 3 817.38 5.00 175 71 75 4 809.72 3.80 176 71 169 98 420.76 29.66 177 71 170 99 420.70 29.66 178 71 173 102 409.98 26.36 179 71 188 117 354.57 27.30 180 71 192 121 351.41 27.89 181 71 205 134 279.44 18.72 182 71 223 152 279.38 18.69 183 72 75 3 807.62 2.25 184 72 76 4 795.94 3.08 185 72 136 64 478.56 22.49 186 72 139 67 478.45 22.55 187 72 141 69 478.16 22.61 188 72 169 97 420.75 29.65 189 73 76 3 807.53 2.71 190 73 77 4 791.49 0.92 191 73 120 47 489.37 25.00 192 73 123 50 489.45 25.07 193 73 124 51 488.99 24.93 194 73 132 59 479.06 22.46 195 74 77 3 805.87 1.74 196 74 78 4 791.51 0.90 197 74 83 9 582.74 25.53 198 74 108 34 489.89 24.99 199 74 112 38 489.88 25.00 200 74 120 46 489.45 25.03 201 74 188 114 354.73 27.34 202 75 78 3 815.20 0.26 203 75 79 4 791.52 0.90 204 75 139 64 478.78 22.69 205 75 169 94 420.81 29.62 206 75 173 98 412.43 27.03 207 75 178 103 357.84 27.05 208 75 180 105 356.82 27.20 209 75 188 113 354.69 27.31 210 76 79 3 809.88 2.40 211 76 80 4 791.50 0.90 212 76 127 51 489.27 25.11 213 76 132 56 486.07 25.82 214 76 135 59 479.29 22.75 215 76 136 60 479.47 22.70 216 76 139 63 478.86 22.64 217 77 80 3 821.92 3.62 218 77 81 4 736.25 26.73 219 77 82 5 654.89 34.02 220 77 83 6 612.27 32.33 221 77 123 46 489.49 25.07 222 77 127 50 489.28 25.11 223 78 81 3 805.26 0.09 224 78 83 5 612.27 32.33 225 78 85 7 535.34 25.80 226 78 108 30 490.43 24.94 227 78 184 106 356.37 27.16 228 78 187 109 356.14 27.21 229 78 188 110 355.63 26.97 230 79 135 56 486.12 25.25 231 79 180 101 357.13 27.04 232 79 181 102 356.81 27.20 233 79 184 105 356.39 27.16 234 80 127 47 489.32 25.14 235 80 131 51 488.75 25.36 236 80 135 55 487.85 24.93 237 82 123 41 489.67 25.07 238 82 126 44 489.58 25.02 239 82 127 45 489.41 25.09 240 82 131 49 489.54 25.02 241 83 88 5 603.14 30.32 242 83 90 7 552.76 38.86 243 83 108 25 492.34 25.17 244 83 123 40 489.67 25.07 245 84 88 4 710.69 19.89 246 85 88 3 828.00 3.38 247 86 108 22 495.39 23.92 248 86 187 101 355.61 26.98 249 88 107 19 496.44 23.74 250 88 108 20 496.40 23.74 251 89 105 16 496.57 23.71 252 89 106 17 496.44 23.74 253 89 107 18 496.44 23.75 254 90 105 15 496.59 23.70 255 90 106 16 496.46 23.74 256 90 108 18 496.45 23.74 257 90 119 29 494.00 24.64 258 90 123 33 491.99 25.64 259 91 105 14 496.70 23.64 260 92 95 3 897.67 1.61 261 92 97 5 740.94 22.96 262 92 103 11 496.82 23.66 263 92 104 12 496.75 23.64 264 92 105 13 496.73 23.64 265 96 103 7 566.00 31.89 266 97 102 5 707.21 19.14 267 97 103 6 579.34 25.21 268 97 118 21 497.69 23.38 269 97 119 22 497.71 23.38 270 98 102 4 815.57 2.56 271 99 102 3 833.10 0.83 272 99 103 4 811.74 0.64 273 100 103 3 816.97 3.25 274 101 104 3 888.14 2.31 275 102 106 4 829.51 0.58 276 102 114 12 516.73 26.12 277 102 115 13 516.73 26.12 278 102 116 14 516.02 25.95 279 102 117 15 516.20 25.87 280 102 118 16 516.19 25.87 281 106 110 4 716.41 23.84 282 106 111 5 716.39 23.84 283 106 114 8 649.52 21.30 284 106 116 10 569.71 23.93 285 106 119 13 552.03 32.51 286 107 110 3 740.86 24.79 287 107 111 4 793.05 5.33 288 108 111 3 815.79 4.46 289 108 112 4 807.00 0.06 290 108 120 12 569.74 23.92 291 108 187 79 355.56 26.99 292 109 112 3 815.80 4.46 293 109 113 4 802.90 2.12 294 109 190 81 354.58 27.30 295 109 191 82 352.78 27.88 296 109 193 84 352.78 27.88 297 110 113 3 809.12 0.99 298 110 114 4 802.88 2.10 299 111 114 3 816.24 2.57 300 111 115 4 783.19 9.61 301 111 116 5 645.84 26.14 302 111 119 8 606.82 37.69 303 111 120 9 631.66 25.26 304 112 115 3 809.10 1.01 305 112 120 8 612.88 34.66 306 112 191 79 352.69 27.84 307 112 192 80 352.64 27.86 308 112 205 93 279.53 18.68 309 113 191 78 352.76 27.88 310 113 192 79 352.66 27.85 311 113 193 80 352.63 27.86 312 113 194 81 351.43 28.12 313 115 205 90 279.34 18.66 314 116 119 3 829.05 1.05 315 116 120 4 812.31 0.29 316 117 120 3 822.07 3.43 317 117 121 4 812.02 0.42 318 117 155 38 473.01 24.06 319 118 121 3 819.90 3.52 320 118 122 4 812.02 0.42 321 119 122 3 816.33 2.56 322 119 123 4 812.02 0.42 323 120 123 3 808.33 3.42 324 120 124 4 807.13 2.83 325 121 124 3 817.22 1.03 326 121 125 4 807.13 2.83 327 121 151 30 474.51 23.48 328 121 154 33 474.50 23.48 329 121 155 34 473.73 23.79 330 122 125 3 820.75 0.72 331 122 126 4 807.10 2.81 332 123 126 3 812.21 0.26 333 123 127 4 805.21 3.75 334 124 127 3 805.21 3.75 335 124 128 4 789.14 6.12 336 124 132 8 708.43 31.07 337 124 150 26 475.44 23.23 338 124 151 27 475.31 23.23 339 124 154 30 475.11 23.25 340 125 128 3 805.20 3.76 341 125 150 25 475.45 23.23 342 125 151 26 475.44 23.23 343 126 131 5 803.99 1.34 344 127 131 4 799.50 3.16 345 127 132 5 793.05 4.46 346 128 131 3 834.21 3.82 347 128 132 4 803.53 1.15 348 128 150 22 475.59 23.30 349 129 132 3 818.07 3.42 350 129 133 4 809.11 2.96 351 129 146 17 478.38 22.69 352 129 150 21 475.61 23.31 353 130 133 3 811.98 1.53 354 130 134 4 809.12 2.96 355 131 134 3 809.12 2.96 356 131 135 4 813.91 0.57 357 132 135 3 821.63 3.29 358 132 136 4 813.91 0.57 359 132 146 14 478.38 22.69 360 132 150 18 475.60 23.31 361 132 153 21 475.48 23.30 362 133 136 3 814.45 0.83 363 133 137 4 813.92 0.57 364 133 144 11 478.56 22.64 365 133 146 13 478.38 22.70 366 134 137 3 813.92 0.57 367 134 138 4 813.91 0.57 368 135 138 3 806.93 1.16 369 135 139 4 801.35 0.46 370 136 139 3 802.01 0.12 371 136 140 4 785.44 4.62 372 136 141 5 689.65 32.45 373 136 144 8 493.44 15.20 374 136 146 10 478.38 22.70 375 136 153 17 475.38 23.33 376 136 159 23 440.31 23.75 377 137 140 3 786.08 4.29 378 137 144 7 528.04 19.98 379 138 179 41 356.88 27.17 380 139 169 30 420.84 29.62 381 139 178 39 356.94 27.15 382 139 179 40 356.88 27.17 383 140 144 4 812.51 0.73 384 140 165 25 436.22 22.92 385 141 144 3 823.10 3.64 386 141 159 18 440.31 23.75 387 141 161 20 437.58 22.70 388 141 169 28 420.82 29.62 389 142 160 18 440.00 23.89 390 142 161 19 437.57 22.69 391 142 165 23 429.01 26.52 392 143 158 15 440.66 23.73 393 143 159 16 440.32 23.74 394 143 160 17 440.01 23.89 395 144 159 15 440.32 23.75 396 145 158 13 440.50 23.80 397 145 159 14 440.32 23.75 398 146 150 4 787.77 6.71 399 146 153 7 517.67 20.21 400 147 153 6 616.76 45.16 401 147 157 10 448.19 20.00 402 147 158 11 440.75 23.71 403 148 152 4 813.52 2.96 404 148 157 9 451.04 21.43 405 149 152 3 825.44 0.62 406 149 153 4 813.21 3.13 407 150 153 3 856.65 1.65 408 150 154 4 856.65 1.65 409 151 154 3 956.20 2.64 410 151 155 4 850.92 1.22 411 152 155 3 829.14 0.58 412 152 156 4 808.71 0.58 413 152 157 5 712.04 30.27 414 153 157 4 608.08 27.89 415 153 158 5 608.08 27.89 416 153 159 6 582.12 14.93 417 154 159 5 582.07 14.90 418 161 165 4 803.71 5.91 419 161 166 5 789.84 5.67 420 161 221 60 278.81 18.98 421 162 165 3 811.31 4.01 422 162 166 4 803.71 5.92 423 162 221 59 278.84 18.97 424 163 166 3 809.41 3.06 425 163 167 4 813.18 1.18 426 163 221 58 278.90 19.00 427 163 236 73 238.79 1.04 428 163 238 75 237.47 0.45 429 164 167 3 813.18 1.18 430 164 168 4 801.13 1.52 431 165 168 3 811.56 0.96 432 165 169 4 801.10 1.51 433 166 169 3 814.73 0.62 434 166 170 4 800.42 1.17 435 166 221 55 279.23 18.84 436 166 223 57 279.25 18.63 437 166 236 70 239.31 1.30 438 167 170 3 815.72 0.13 439 167 171 4 800.42 1.17 440 167 234 67 240.34 0.96 441 167 235 68 240.16 0.99 442 167 236 69 239.55 0.75 443 168 171 3 815.74 0.12 444 168 172 4 813.46 1.01 445 169 172 3 813.47 1.01 446 169 173 4 813.46 1.01 447 169 178 9 600.03 25.00 448 170 173 3 810.37 4.93 449 170 174 4 795.14 0.50 450 170 223 53 279.56 18.66 451 170 224 54 279.43 18.58 452 170 225 55 279.47 18.54 453 170 235 65 240.36 0.98 454 171 174 3 807.19 6.52 455 171 175 4 807.18 6.52 456 171 234 63 240.43 0.96 457 172 175 3 820.38 0.08 458 172 176 4 792.39 0.88 459 172 177 5 619.04 34.48 460 172 178 6 605.89 27.93 461 173 176 3 810.33 4.94 462 173 178 5 673.40 12.01 463 173 185 12 355.54 27.53 464 173 189 16 354.87 27.29 465 173 192 19 353.97 27.44 466 173 225 52 279.88 18.46 467 174 189 15 354.87 27.29 468 174 225 51 279.91 18.46 469 174 227 53 280.06 18.28 470 174 232 58 241.10 1.16 471 174 234 60 240.84 1.08 472 176 182 6 438.69 13.96 473 176 185 9 438.51 13.92 474 176 189 13 355.50 27.37 475 177 185 8 510.55 21.79 476 178 185 7 563.23 26.37 477 180 185 5 794.10 2.93 478 181 184 3 810.95 4.16 479 181 185 4 802.84 1.34 480 182 185 3 810.95 4.17 481 182 186 4 802.84 1.34 482 183 186 3 806.51 1.94 483 183 187 4 804.09 0.71 484 184 187 3 809.74 3.54 485 184 188 4 804.11 0.69 486 185 188 3 819.20 2.92 487 185 189 4 799.88 2.99 488 186 189 3 812.12 3.20 489 186 190 4 802.89 0.07 490 187 190 3 819.37 1.78 491 187 191 4 802.89 0.07 492 188 191 3 809.30 3.26 493 188 192 4 809.30 3.27 494 189 192 3 811.00 2.42 495 189 193 4 808.32 3.75 496 189 203 14 280.76 18.92 497 189 225 36 280.07 18.39 498 190 193 3 812.73 0.48 499 192 203 11 280.70 18.94 500 192 204 12 280.39 19.01 501 192 205 13 280.34 18.98 502 193 196 3 881.44 2.46 503 193 201 8 281.36 18.93 504 193 202 9 281.17 18.96 505 193 203 10 280.71 18.93 506 194 202 8 281.25 18.96 507 194 204 10 280.85 18.87 508 195 200 5 673.25 35.91 509 195 201 6 423.30 89.89 510 195 202 7 281.36 18.91 511 196 200 4 663.19 56.43 512 196 201 5 423.33 89.88 513 197 200 3 796.24 0.51 514 199 228 29 543.59 113.41 515 199 229 30 543.57 113.42 516 200 226 26 543.92 113.47 517 200 227 27 543.82 113.51 518 200 228 28 543.60 113.41 519 200 263 63 100.09 0.15 520 200 264 64 97.90 0.58 521 200 265 65 92.13 1.14 522 201 226 25 543.88 113.49 523 201 227 26 543.83 113.52 524 202 225 23 543.92 113.49 525 202 226 24 543.84 113.52 526 203 224 21 544.01 113.48 527 203 225 22 543.89 113.52 528 204 222 18 544.15 113.45 529 204 223 19 544.12 113.45 530 204 224 20 544.02 113.48 531 204 235 31 504.67 133.12 532 205 222 17 544.16 113.45 533 205 223 18 544.14 113.45 534 206 221 15 544.19 113.44 535 206 222 16 544.16 113.45 536 206 250 44 117.02 1.96 537 206 251 45 116.94 1.97 538 207 220 13 544.32 113.48 539 207 221 14 544.27 113.48 540 207 250 43 117.03 1.97 541 208 219 11 544.57 113.41 542 208 220 12 544.33 113.47 543 208 241 33 503.32 133.39 544 208 244 36 455.60 123.00 545 208 247 39 120.17 2.35 546 208 250 42 117.12 1.99 547 209 218 9 544.69 113.45 548 209 219 10 544.62 113.44 549 210 218 8 544.75 113.41 550 210 241 31 503.68 133.54 551 211 217 6 544.94 113.37 552 211 218 7 544.82 113.38 553 211 241 30 503.68 133.54 554 211 242 31 502.99 133.46 555 211 243 32 502.97 133.47 556 212 216 4 758.45 6.64 557 212 217 5 544.94 113.36 558 212 218 6 544.91 113.37 559 213 216 3 765.75 3.00 560 213 217 4 685.98 42.88 561 213 242 29 503.76 133.55 562 214 242 28 503.79 133.53 563 217 240 23 503.81 133.54 564 217 241 24 503.76 133.55 565 217 242 25 503.66 133.56 566 218 240 22 503.76 133.55 567 218 241 23 503.69 133.55 568 219 238 19 503.83 133.54 569 219 239 20 503.75 133.56 570 219 241 22 503.54 133.59 571 220 238 18 503.76 133.55 572 220 239 19 503.70 133.54 573 220 241 21 502.97 133.47 574 220 247 27 117.08 1.99 575 220 250 30 116.38 2.02 576 221 237 16 503.79 133.53 577 221 238 17 503.62 133.55 578 221 250 29 111.44 1.56 579 222 235 13 504.58 133.18 580 222 236 14 504.05 133.41 581 222 237 15 503.75 133.50 582 222 250 28 111.52 1.59 583 222 251 29 111.42 1.56 584 222 254 32 109.06 0.52 585 222 255 33 109.05 0.52 586 222 258 36 104.76 0.95 587 223 235 12 504.56 133.18 588 223 236 13 504.43 133.19 589 224 234 10 504.72 133.10 590 224 235 11 504.62 133.12 591 225 232 7 504.96 133.10 592 225 233 8 504.83 133.05 593 225 234 9 504.65 133.06 594 225 235 10 504.65 133.06 595 226 232 6 504.97 133.10 596 226 233 7 504.95 133.09 597 226 235 9 504.75 133.02 598 226 258 32 105.73 1.23 599 226 263 37 93.11 0.69 600 227 231 4 712.45 29.46 601 227 232 5 504.95 133.09 602 228 231 3 794.70 0.45 603 228 262 34 101.84 0.19 604 228 263 35 99.30 0.15 605 228 264 36 97.65 0.51 606 228 265 37 92.28 1.10 607 233 263 30 92.23 1.12 608 235 258 23 101.08 0.14 609 237 250 13 111.40 1.55 610 237 253 16 108.98 0.55 611 237 254 17 105.84 1.18 612 239 246 7 635.26 1.26 613 239 249 10 115.56 2.17 614 239 250 11 110.77 1.24 615 241 244 3 874.89 0.42 616 241 246 5 757.20 11.10 617 241 247 6 607.18 15.45 618 243 246 3 833.46 2.64 619 244 247 3 826.81 0.69 620 245 248 3 902.12 2.02 621 246 249 3 834.21 2.94 622 247 250 3 824.05 1.30 623 248 251 3 828.26 0.03 624 248 252 4 798.00 0.77 625 249 252 3 797.87 0.84 626 249 253 4 794.48 2.53 627 249 254 5 797.30 1.22 628 250 254 4 810.73 5.50 629 251 254 3 820.74 0.50 630 251 255 4 798.10 0.82 631 252 255 3 810.73 5.50 632 252 256 4 798.10 0.81 633 253 256 3 811.02 5.36 634 253 257 4 790.38 2.29 635 254 257 3 797.64 1.34 636 254 258 4 787.42 3.77 637 255 258 3 800.72 0.20 638 255 259 4 786.82 0.20 639 256 259 3 812.05 5.47 640 256 260 4 793.94 3.25 641 257 260 3 805.22 8.88 642 257 261 4 795.32 3.94 643 258 261 3 795.32 3.94 644 258 262 4 795.15 4.01 645 258 263 5 749.94 13.60 646 259 262 3 795.14 4.02 647 259 263 4 786.69 0.16 648 259 264 5 749.94 13.60